Section-lab

A fresh frozen section of 7-day old mouse
(4 micrometer thick)　マウス全身凍結切片

A fresh frozen section of adult rat thighbone
(4 micrometer thick)　成熟ラット大腿骨凍結切片

A frozen section of pregnant mouse (6 micrometer thick)

SECTION-LAB Co. Ltd.
Hiroshima, Japan

This method produces thin complete frozen sections
from the following specimens.

Revised: Oct/21/2011

川本忠文博士によって考案され、35年かけて実用化された動物試料や植物試料から高品質の凍結切片を作製する方法の事です。マウス全身や骨等からも凍結切片を作製でき、研究や術中迅速組織診断用の切片作製に利用されています。
「川本法」は、これまで不可能とされていた研究の扉を開け、生命科学の研究に大きく貢献しています。

<Official HP of Kawamoto's method>

Applicable specimens
Hard tissue	bone, dentine, human tooth, etc.
Large specimens	whole mouse, child whole rat, etc.
Soft tissue	brain, lung, muscle, kidney, eye, nail, cartilage, etc.
Plants	rice, beans, wheat, pollens, leaves, roots, etc.
Fish	zebrafish, eels, killifish, etc.
Insects	spiders, silkworms, cockroaches, fruit flies, honeybees, et al.
Others	snails, crabs, shells, etc.
Food	noodles, chocolate, etc.

Section applications
Histological staining	Hematoxilin-eosin, Toluidine blue, etc.
Histochemical staining	Arizaline red, Saflanine-O, GBHA staining, etc.
Enzyme histochemistry	ALPase, ACPase, TRAPase, Glycosidase, etc.
Immunohistochemistry	ABC staining
Fluorescence immunohistochemistry	DAPI, FITC, Texas red, etc.
Observation of fluorescence	GFP, Calcein, Tetracycline, Arizaline red, Rhodamine, etc.
In situ hybridization	ISH, FISH
Gene analysis (LMD)	using Laser microdisection technique
Mass imaging
EPMA	Elemental analysis, Back scatter electron image, etc.
Autoradiography	Water soluble materials